Prosphere 300 C4 is a silica-based HPLC column with a butyl bonded phase on wide-pore (300 Å) silica, specifically designed for the separation of peptides, proteins, and other biomacromolecules. The shorter alkyl chains provide reduced hydrophobic retention compared to C18 or C8 phases, enabling faster elution and gentler interactions with large analytes.
The wide-pore structure ensures excellent accessibility for high-molecular-weight compounds, while the robust bonding chemistry provides sharp peak shapes, reproducibility, and long column lifetimes. Prosphere 300 C4 is an excellent choice for biopharmaceutical, proteomic, and biochemical applications requiring reliable biomolecular separations.
Prosphere 300 C4 provides gentle hydrophobic selectivity with wide-pore accessibility – the reliable choice for laboratories analyzing peptides and proteins in HPLC.
Wide-pore silica stationary phase (300 Å) with butyl (C4) bonded chemistry
Reduced hydrophobic retention compared to C18 and C8 phases
Optimized for peptides, proteins, and other biomacromolecules
High reproducibility and robust operational lifetime
Available particle sizes: 3 µm, 5 µm, 10 µm
Separation of peptides and proteins in pharmaceutical research
Biopharmaceutical testing of therapeutic proteins and biomolecules
Proteomic analysis requiring mild hydrophobic interactions
Method development for wide-pore separations with reduced retention
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 6 % |
| pH Range | 2 to 7 |
| Phase | Material Number |
|---|---|
| Prosphere 300 C4, 3 µm | 5202026 |
| Prosphere 300 C4, 5 µm | 5202025 |
| Prosphere 300 C4, 10 µm | 5202024 |