Prosphere 300 C8 is a silica-based HPLC column with an octyl bonded phase on wide-pore (300 Å) silica, developed for the analysis of peptides, proteins, and other biomacromolecules. The medium hydrophobic retention of the C8 chains provides a balance between the strong retention of C18 and the milder interactions of C4 phases, offering versatile selectivity for complex biomolecular samples.
The wide-pore structure ensures accessibility for high-molecular-weight analytes, while robust bonding chemistry delivers sharp peak shapes, reproducibility, and long operational lifetimes. Prosphere 300 C8 is particularly suited for biopharmaceutical and proteomic laboratories where balanced retention and reliability are essential.
Prosphere 300 C8 provides balanced retention with wide-pore accessibility – the dependable choice for laboratories analyzing peptides, proteins, and biomolecules in HPLC.
Wide-pore silica stationary phase (300 Å) with octyl (C8) bonded chemistry
Medium hydrophobic retention for balanced separations
Optimized for peptides, proteins, and biomacromolecules
High reproducibility and long operational lifetime
Available particle sizes: 3 µm, 5 µm, 10 µm
Separation of peptides and proteins in pharmaceutical and proteomic research
Biopharmaceutical testing of therapeutic proteins and biomolecules
Proteomic analysis requiring balanced hydrophobic interactions
Method development for biomolecular separations on wide-pore silica
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C8 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 8 % |
| pH Range | 2 to 7 |
| Phase | Material Number |
|---|---|
| Prosphere 300 C8, 3 µm | 5202027 |
| Prosphere 300 C8, 5 µm | 5202022 |
| Prosphere 300 C8, 10 µm | 5202023 |