Prosphere 300 Phenyl is a silica-based HPLC column with a phenyl bonded phase on wide-pore (300 Å) silica, specifically developed for the separation of peptides, proteins, and other biomacromolecules. The phenyl functionality introduces π–π interactions and enhanced aromatic selectivity, providing complementary retention compared to conventional alkyl phases such as C18 or C8.
The wide-pore structure ensures excellent accessibility for large analytes, while the phenyl chemistry improves resolution of aromatic and structurally similar biomolecules. With robust bonding chemistry, Prosphere 300 Phenyl delivers sharp peak shapes, reproducibility, and long operational lifetimes.
Prosphere 300 Phenyl combines wide-pore accessibility with aromatic selectivity – the dependable choice for laboratories analyzing peptides, proteins, and biomolecules with HPLC.
Wide-pore silica stationary phase (300 Å) with phenyl bonded chemistry
π–π interactions for improved separation of aromatic biomolecules
Optimized for peptides, proteins, and other large analytes
High reproducibility and robust operational lifetime
Available particle sizes: 3 µm, 5 µm, 10 µm
Separation of aromatic peptides and proteins in pharmaceutical and proteomic research
Biochemical analysis of aromatic amino acids and conjugated biomolecules
Pharmaceutical impurity profiling requiring aromatic selectivity
Method development requiring orthogonal selectivity to C18 and C8 phases
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | Phenyl |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| pH Range | 2 to 7 |
| Phase | Material Number |
|---|---|
| Prosphere 300 Phenyl, 3 µm | 5210045 |
| Prosphere 300 Phenyl, 5 µm | 5210046 |
| Prosphere 300 Phenyl, 10 µm | 5210047 |