Prosphere 300 C18 is a silica-based HPLC column with an octadecyl bonded phase on wide-pore (300 Å) silica, specifically designed for the separation of peptides, proteins, and other biomacromolecules. The long C18 chains provide strong hydrophobic retention, ensuring reproducibility, sharp peak shapes, and reliable performance for large analytes.
The wide-pore structure offers excellent accessibility for high-molecular-weight compounds, while robust bonding chemistry ensures long operational lifetimes under routine and demanding chromatographic conditions. Prosphere 300 C18 is the standard choice for laboratories performing biomolecular reversed-phase separations.
Prosphere 300 C18 delivers strong hydrophobic retention with wide-pore accessibility – the dependable choice for laboratories analyzing proteins, peptides, and biomolecules in HPLC.
Wide-pore silica stationary phase (300 Å) with octadecyl (C18) bonded chemistry
Strong hydrophobic retention for peptides, proteins, and biomolecules
Optimized for biomacromolecules requiring wide-pore accessibility
High reproducibility and robust column lifetime
Available particle sizes: 3 µm, 5 µm, 10 µm
Separation of peptides and proteins in proteomic research
Biopharmaceutical analysis of therapeutic proteins and large biomolecules
Pharmaceutical impurity profiling of peptide-based drugs
Method development requiring robust wide-pore C18 selectivity
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 13 % |
| pH Range | 2 to 7 |
| Phase | Material Number |
|---|---|
| Prosphere 300 C18, 3 µm | 5202021 |
| Prosphere 300 C18, 5 µm | 5202020 |
| Prosphere 300 C18, 10 µm | 5210030 |