Prosphere HP 300 C18-AQ is a high-purity silica-based stationary phase with an octadecyl (C18) bonded surface and additional polar functional groups, developed for aqueous reversed-phase chromatography of proteins, peptides, and polar biomolecules. The 300 Å pore size ensures excellent accessibility and mass transfer for large analytes, while the polar surface modification enhances phase wetting and retention in highly aqueous mobile phases.
This polar-embedded C18 chemistry prevents phase collapse in water-rich conditions and improves selectivity for hydrophilic regions of biomolecules. It provides a balanced interaction profile — combining strong hydrophobic retention with controlled polar interactions — making it ideal for separations where conventional C18 phases show poor retention or reduced reproducibility.
The high-purity silica backbone guarantees mechanical stability, low background activity, and excellent peak symmetry, even under demanding gradient and high-pressure conditions. Prosphere HP 300 C18-AQ is particularly suited for biopharmaceutical, proteomic, and peptide analysis applications requiring robust performance and enhanced retention of polar compounds.
Prosphere HP 300 C18-AQ combines the strong retention of a C18 phase with enhanced polar selectivity and aqueous stability – the reliable choice for high-performance separations of polar and hydrophilic biomolecules under reversed-phase conditions.
High-purity silica stationary phase with 300 Å pore size and polar-modified C18 bonding
Stable under highly aqueous mobile phase conditions (no phase collapse)
Combines hydrophobic and polar selectivity for complex biomolecules
High reproducibility, peak symmetry, and long column lifetime
Suitable for analytical and preparative HPLC applications
Separation of polar peptides, proteins, and hydrophilic biomolecules
Peptide mapping and analysis of protein digests in aqueous media
Characterization of biopharmaceutical compounds and nucleic acid fragments
Quality control and research in pharmaceutical and biochemical laboratories
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C18-AQ |
| Particle Type | |
| Separation Modes | |
| USP Codes | |
| Endcapping | Yes |
| Carbonload | 14 % |
| pH Range | 2 to 7 |
| Phase | Material Number |
|---|---|
| Prosphere HP 300 C18-AQ, 3 µm | 5210051 |
| Prosphere HP 300 C18-AQ, 5 µm | 5210052 |
| Prosphere HP 300 C18-AQ, 10 µm | 5210053 |