Prosphere HP 300 C18 is a high-purity silica-based HPLC stationary phase with an octadecyl bonded surface and a 300 Å pore size, specifically engineered for the reversed-phase separation of proteins, peptides, and nucleic acid fragments. The long C18 chains provide strong hydrophobic retention, delivering high resolution and excellent peak symmetry for complex biomolecular mixtures.
The large-pore structure of 300 Å ensures efficient mass transfer and optimal accessibility for high-molecular-weight compounds such as peptides, protein fragments, and biomolecules. The high-purity silica matrix minimizes secondary interactions and adsorption effects, ensuring reproducible retention and consistent recovery — even under demanding gradient and aqueous-organic mobile phase conditions.
Prosphere HP 300 C18 is ideal for applications requiring strong hydrophobic selectivity and high efficiency, such as peptide mapping, protein characterization, and biopharmaceutical analysis, where precise separation and structural integrity are critical.
Prosphere HP 300 C18 combines strong hydrophobic selectivity with high-purity, large-pore silica technology – the reliable choice for reproducible and high-resolution separations of proteins, peptides, and other complex biomolecules.
High-purity silica stationary phase with 300 Å pore size and C18 bonding
Strong hydrophobic retention for proteins, peptides, and nucleic acid fragments
Excellent mass transfer and resolution for large biomolecules
High reproducibility and long column lifetime under gradient conditions
Suitable for analytical and preparative biopharmaceutical workflows
Available particle sizes: 3 µm, 5 µm, 10 µm
Peptide mapping and protein fragment analysis
Characterization of therapeutic proteins and biopharmaceuticals
Separation of hydrophobic peptides and nucleic acid fragments
Quality control and method development in life-science and pharmaceutical research
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 13.5 % |
| pH Range | 2 to 7 |
| Phase | Material Number |
|---|---|
| Prosphere HP 300 C18, 3 µm | 5210048 |
| Prosphere HP 300 C18, 5 µm | 5210049 |
| Prosphere HP 300 C18, 10 µm | 5210050 |