Prosphere HP 300 C4 is a high-purity silica-based HPLC stationary phase with a butyl bonded surface and a 300 Å pore size, specifically designed for the separation of proteins, peptides, and other large biomolecules. The shorter C4 alkyl chains provide moderate hydrophobic retention, enabling gentle and controlled interactions ideal for high-molecular-weight and partially hydrophobic analytes.
The large 300 Å pore structure ensures excellent accessibility and mass transfer for biomolecules, while the high-purity silica matrix minimizes non-specific interactions and adsorption. This results in sharp peak shapes, high resolution, and reproducible retention — even for complex biological samples or gradient separations. Prosphere HP 300 C4 delivers exceptional performance and stability under aqueous-organic conditions, making it particularly suited for protein characterization, peptide mapping, and biopharmaceutical analysis where both resolution and sample recovery are critical.
Prosphere HP 300 C4 combines controlled hydrophobic selectivity with a large-pore, high-purity silica matrix – the reliable choice for precise, reproducible, and gentle separations of proteins and peptides.
High-purity silica stationary phase with 300 Å pore size and C4 bonding
Optimized for proteins, peptides, and other biomolecules
Moderate hydrophobic retention for gentle separation conditions
High efficiency, reproducibility, and column lifetime
Suitable for analytical and preparative biopharmaceutical workflows
Available particle sizes: 3 µm, 5 µm, 10 µm
Separation and purification of proteins and peptides
Peptide mapping and enzymatic digest analysis
Characterization of recombinant and therapeutic proteins
Biopharmaceutical research and quality control
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 5 % |
| pH Range | 2 to 7 |
| Phase | Material Number |
|---|---|
| Prosphere HP 300 C4, 3 µm | 5210024 |
| Prosphere HP 300 C4, 5 µm | 5210054 |
| Prosphere HP 300 C4, 10 µm | 5210055 |