Prosphere HP 300 C8 is a high-purity silica-based HPLC stationary phase with an octyl bonded surface and a 300 Å pore size, developed for the reversed-phase separation of proteins, peptides, and other large biomolecules. The medium-chain C8 bonding offers balanced hydrophobic retention, making it ideal for biomolecules that require stronger interactions than C4 phases while maintaining excellent recovery and resolution.
The wide 300 Å pore structure provides optimal accessibility and diffusion for macromolecules, ensuring efficient mass transfer and sharp peak shapes. The high-purity silica matrix minimizes secondary interactions and adsorption, enabling reproducible separations even for sensitive biomolecules or gradient elution methods.
With its outstanding mechanical stability and consistent retention behavior, Prosphere HP 300 C8 is perfectly suited for biochemical, pharmaceutical, and proteomic applications, including peptide mapping, protein fragment analysis, and the characterization of therapeutic biomolecules.
Prosphere HP 300 C8 combines balanced hydrophobic selectivity with large-pore, high-purity silica technology – the dependable choice for reproducible and high-resolution separations of peptides and proteins.
High-purity silica stationary phase with 300 Å pore size and C8 bonding
Balanced hydrophobic retention for proteins, peptides, and biomolecules
Excellent mass transfer and peak symmetry for large molecules
High reproducibility and robustness under gradient conditions
Suitable for analytical and preparative HPLC applications
Available particle sizes: 3 µm, 5 µm, 10 µm
Separation of peptides, proteins, and biomolecular fragments
Peptide mapping and characterization of biopharmaceuticals
Analysis of hydrophobic and amphiphilic biomolecules
Quality control and research in proteomics and biotechnology
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C8 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| pH Range | 2 to 7 |
| Phase | Material Number |
|---|---|
| Prosphere HP 300 C8, 3 µm | 5210033 |
| Prosphere HP 300 C8, 5 µm | 5210056 |
| Prosphere HP 300 C8, 10 µm | 5210057 |