ReproSil Chiral-L-Hydroxy-Prolin is a silica-based HPLC stationary phase functionalized with the natural amino acid L-hydroxyproline, developed for enantioselective separations based on ligand-exchange, hydrogen bonding, and ionic interactions. The immobilized L-hydroxyproline provides a well-defined chiral environment capable of forming transient diastereomeric complexes with analytes containing carbonyl, hydroxyl, or amino groups near the stereogenic center.
The presence of both hydroxyl and secondary amine functional groups in L-hydroxyproline allows multiple interaction mechanisms, including acid–base interactions, metal coordination, and hydrogen bonding, giving the phase a broad selectivity range for polar and biologically relevant compounds. This makes ReproSil Chiral-L-Hydroxy-Prolin particularly effective for the resolution of amino acids, peptides, α-hydroxy acids, and other polar chiral molecules.
The chiral selector is covalently attached to high-purity spherical silica, ensuring high mechanical stability, reproducibility, and long column lifetime. The phase can be operated under aqueous, polar-organic, or ligand-exchange conditions, providing versatility in method development and compatibility with various analytical requirements. ReproSil Chiral-L-Hydroxy-Prolin is ideally suited for pharmaceutical, biochemical, and environmental laboratories that require reliable and reproducible chiral separations of polar compounds and biomolecule derivatives.
ReproSil Chiral-L-Hydroxy-Prolin utilizes the intrinsic chirality and coordinating functionality of L-hydroxyproline to achieve precise and reproducible enantioselective separations – the reliable choice for polar and biologically relevant compounds in advanced chiral chromatography.
Silica-based stationary phase modified with covalently bound L-hydroxyproline
Chiral recognition through hydrogen bonding, ionic, and ligand-exchange mechanisms
Ideal for polar chiral compounds such as amino acids, peptides, and α-hydroxy acids
High chemical and mechanical stability with excellent reproducibility
Operates under aqueous, polar-organic, or ligand-exchange conditions
Suitable for analytical and preparative chiral HPLC applications
Enantioselective separation of amino acids, peptides, and hydroxy acids
Resolution of biologically active chiral molecules and natural products
Method development for ligand-exchange and polar-organic chiral separations
Stereochemical purity analysis and quality control in pharmaceutical and biochemical research
| Particle Size |
|
|---|---|
| Pore Size | 100 Å |
| Surface Area | 350 m²/g |
| Phase/Modification | L-Hydroxy-Prolin |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil Chiral-L-Hydroxy-Prolin, 5 µm | r15.hp. |