ReproSil-Pur 1000 NH2 is a silica-based HPLC column with bonded amino functionality on high-purity 1000 Å silica. The ultra-wide pore structure provides excellent accessibility for very large biomolecules, while the amino surface enables strong hydrogen bonding and dipole–dipole interactions, making it particularly effective for carbohydrate and glycan analysis.
Compared to narrower-pore amino columns, ReproSil-Pur 1000 NH2 allows improved mass transfer for polysaccharides, glycoproteins, and other high-molecular-weight analytes. The robust bonding chemistry ensures reproducibility, sharp peak shapes, and long operational lifetimes.
ReproSil-Pur 1000 NH2 combines ultra-wide-pore accessibility with amino functionality – the dependable choice for laboratories analyzing carbohydrates and very large biomolecules by HPLC.
High-purity silica with amino (NH₂) bonded chemistry
1000 Å ultra-wide pore size for very large biomolecules
Strong hydrogen bonding and dipole–dipole interactions
Optimized for carbohydrates, glycans, and polysaccharides
Available particle sizes: 3 µm, 5 µm
Carbohydrate and glycan analysis in biopharmaceutical research
Separation of polysaccharides, glycopeptides, and glycoproteins
Biochemical studies of nucleosides, sugars, and polar biomolecules
Method development requiring orthogonal selectivity to C18 and other phases
| Particle Sizes |
|
|---|---|
| Pore Size | 1 000 Å |
| Surface Area | 25 m²/g |
| Phase/Modification | NH2 |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | No |
| Carbonload | 1 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 1000 NH2, 3 µm | r63.a0. |
| ReproSil-Pur 1000 NH2, 5 µm | r65.a0. |