ReproSil-Pur 1000 ODS-3

Ultra-Wide-Pore C18 Column for Intact Proteins and Large Biomolecules

ReproSil-Pur 1000 ODS-3 is a silica-based HPLC column with octadecyl bonded chemistry on high-purity 1000 Å silica. The ultra-wide pore size provides excellent accessibility for very large biomolecules such as intact proteins, antibodies, glycoproteins, and nucleic acids, while the C18 bonding ensures strong hydrophobic retention and reliable separations.

Compared to narrower-pore C18 phases, ReproSil-Pur 1000 ODS-3 offers improved mass transfer and resolution for ultra-large analytes, making it a powerful tool for biopharmaceutical and proteomics applications. Robust bonding chemistry ensures reproducibility, sharp peak shapes, and long column lifetimes under gradient and high-temperature conditions.

ReproSil-Pur 1000 ODS-3 combines ultra-wide-pore accessibility with proven C18 chemistry – the reliable choice for laboratories analyzing intact proteins and other large biomolecules by HPLC.

Key Features:

  • High-purity silica with octadecyl (C18, ODS) bonded chemistry

  • 1000 Å ultra-wide-pore structure for intact proteins and large biomolecules

  • Strong hydrophobic retention with reproducible peak shapes

  • Robust bonding chemistry for long operational lifetimes

  • Available particle sizes: 5 µm, 10 µm

Typical Applications:

  • Biopharmaceutical analysis of intact proteins, antibodies, and glycoproteins

  • Proteomics workflows requiring ultra-wide-pore reversed-phase columns

  • Separation of nucleic acids, oligonucleotides, and large biomolecular complexes

  • Method development requiring C18 selectivity for very large analytes

Specifications

Request Product
Particle Sizes
  • 5 µm
  • 10 µm
Pore Size 1 000 Å
Surface Area 25 m²/g
Phase/Modification C18
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 1.6 %
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil-Pur 1000 ODS-3, 5 µm r65.93.
ReproSil-Pur 1000 ODS-3, 10 µm r60.93.
ReproSil-Pur 1000 NH2 ReproSil-Pur 1000 Si

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