ReproSil-Pur 1000 Si is an unbonded, high-purity silica HPLC column with a 1000 Å ultra-wide pore structure, designed for normal-phase and adsorption chromatography of very large biomolecules. The extensive pore system ensures excellent accessibility for intact proteins, antibodies, nucleic acids, and polysaccharides, while the active silanol surface provides strong polar interactions.
Compared to conventional silica phases, ReproSil-Pur 1000 Si allows improved mass transfer and resolution for ultra-large analytes, making it particularly valuable for biopharmaceutical and biochemical applications. Its high-purity silica guarantees reproducibility, sharp peak shapes, and robustness under demanding conditions.
ReproSil-Pur 1000 Si combines ultra-wide-pore silica with strong polar selectivity – the dependable choice for laboratories analyzing very large biomolecules by normal-phase HPLC.
Unbonded, high-purity silica stationary phase
1000 Å ultra-wide pore size for very large biomolecules
Strong polar interactions for normal-phase chromatography
Excellent reproducibility and sharp peak shapes
Available particle size: 5 µm
Normal-phase separations of intact proteins, antibodies, and glycoproteins
Biopharmaceutical analysis of nucleic acids, oligonucleotides, and polysaccharides
Biochemical separations requiring polar selectivity for ultra-large analytes
Method development with orthogonal selectivity to reversed-phase wide-pore phases
| Particle Size |
|
|---|---|
| Pore Size | 1 000 Å |
| Surface Area | 25 m²/g |
| Phase/Modification | Si |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | No |
| Carbonload | 0 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 1000 Si, 5 µm | r65.00. |