ReproSil-Pur 120 Diol

HILIC and Size-Based HPLC Column with Diol Chemistry

ReproSil-Pur 120 Diol is a silica-based HPLC column with bonded diol functionality on high-purity 120 Å silica. The diol surface provides controlled hydrophilicity, making the column highly suitable for hydrophilic interaction chromatography (HILIC) as well as size-based separations of small molecules.

Compared to bare silica, the bonded diol layer reduces strong silanol interactions while maintaining polar selectivity, resulting in improved reproducibility and peak symmetry. This makes ReproSil-Pur 120 Diol an excellent tool for pharmaceutical, biochemical, food, and environmental laboratories working with polar analytes.

ReproSil-Pur 120 Diol delivers reliable polar selectivity with improved reproducibility – the versatile choice for laboratories requiring HILIC and normal-phase HPLC separations.

Key Features:

  • High-purity silica with bonded diol functionality

  • 120 Å pore size optimized for small to medium-sized molecules

  • Suitable for HILIC and normal-phase applications

  • Reduced silanol activity compared to bare silica for better peak shapes

  • Available particle sizes: 3 µm, 5 µm, 10 µm

Typical Applications:

  • Pharmaceutical analysis of hydrophilic drugs and metabolites

  • Biochemical testing of sugars, nucleosides, and polar compounds

  • Food and beverage analysis of carbohydrates and organic acids

  • Environmental monitoring of polar pollutants

  • Method development requiring hydrophilic interaction separations

Specifications

Request Product
Particle Sizes
  • 3 µm
  • 5 µm
  • 10 µm
Pore Size 120 Å
Surface Area 300 m²/g
Phase/Modification Diol
Particle Type
Separation Modes
USP Code
Endcapping No
Carbonload 7 %
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil-Pur 120 Diol, 3 µm r13.d0.
ReproSil-Pur 120 Diol, 5 µm r15.d0.
ReproSil-Pur 120 Diol, 10 µm r10.d0.
ReproSil-Pur 120 CN ReproSil-Pur 120 NH2

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