ReproSil-Pur 200 Si is an unbonded, high-purity silica HPLC column with a 200 Å wide-pore structure, designed for normal-phase and adsorption chromatography of peptides, small proteins, and other medium-sized biomolecules. The large pore size ensures excellent accessibility for biomolecules, while the active silanol surface provides strong polar interactions.
Compared to narrow-pore silica, ReproSil-Pur 200 Si offers improved mass transfer and resolution for biomolecules, delivering reproducible results and sharp peak shapes. It is an excellent tool for laboratories requiring robust and selective normal-phase separations.
ReproSil-Pur 200 Si combines high-purity wide-pore silica with strong polar retention – the reliable choice for laboratories analyzing peptides and biomolecules under normal-phase HPLC conditions.
Unbonded, high-purity silica stationary phase
200 Å wide-pore structure for peptides and medium biomolecules
Strong polar interactions for normal-phase chromatography
Excellent reproducibility with sharp and symmetrical peaks
Available particle sizes: 3 µm, 5 µm
Normal-phase separations of peptides, small proteins, and polar biomolecules
Biopharmaceutical impurity profiling and structural studies
Chemical and biochemical separations requiring polar selectivity
Method development with orthogonal selectivity to reversed-phase HPLC
| Particle Sizes |
|
|---|---|
| Pore Size | 200 Å |
| Surface Area | 200 m²/g |
| Phase/Modification | Si |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | No |
| Carbonload | 0 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 200 Si, 3 µm | r23.00. |
| ReproSil-Pur 200 Si, 5 µm | r25.00. |