ReproSil-Pur 300 C4 is a silica-based HPLC column with butyl bonded chemistry on high-purity 300 Å silica. The wide-pore structure provides excellent accessibility for large biomolecules such as proteins and peptides, while the short-chain alkyl bonding offers reduced hydrophobic retention compared to C8 or C18 phases. This combination enables efficient separations of sensitive biomolecules under milder chromatographic conditions.
The robust bonding chemistry ensures reproducibility, sharp peak shapes, and long operational lifetimes, even under gradient and elevated temperature conditions. ReproSil-Pur 300 C4 is particularly well suited for biopharmaceutical and proteomic laboratories requiring gentle but effective reversed-phase separations.
ReproSil-Pur 300 C4 combines wide-pore accessibility with balanced hydrophobic retention – the reliable choice for laboratories analyzing proteins and peptides by HPLC.
High-purity silica with butyl (C4) bonded chemistry
300 Å wide-pore structure for proteins, peptides, and other large biomolecules
Reduced hydrophobic retention compared to longer alkyl phases
Robust bonding chemistry ensures reproducibility and durability
Available particle sizes: 3 µm, 5 µm, 10 µm
Protein and peptide separations in biopharmaceutical analysis
Proteomics applications requiring wide-pore reversed-phase columns
Biochemical analysis of sensitive biomolecules under mild retention conditions
Method development requiring complementary selectivity to C8 and C18 phases
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 2.5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 300 C4, 3 µm | r33.4e. |
| ReproSil-Pur 300 C4, 5 µm | r35.4e. |
| ReproSil-Pur 300 C4, 10 µm | r30.4e. |