ReproSil-Pur 300 C8 is a silica-based HPLC column with octyl bonded chemistry on high-purity 300 Å silica. The wide-pore design ensures excellent accessibility for larger biomolecules such as peptides and proteins, while the C8 bonding provides medium hydrophobic retention – shorter than C18 but stronger than C4. This balance makes it highly versatile for biomolecular separations.
The robust bonding chemistry delivers reproducible results, sharp peak shapes, and long column lifetimes, even under demanding gradient or elevated temperature conditions. ReproSil-Pur 300 C8 is widely used in biopharmaceutical, biochemical, and proteomics applications.
ReproSil-Pur 300 C8 provides wide-pore accessibility with balanced hydrophobic retention – the reliable choice for laboratories analyzing proteins and peptides by HPLC.
High-purity silica with octyl (C8) bonded chemistry
300 Å wide-pore structure for proteins, peptides, and biomolecules
Medium hydrophobic retention, ideal for biomolecules sensitive to strong retention
Robust bonding chemistry for reproducibility and durability
Available particle sizes: 3 µm, 5 µm, 10 µm, 15 µm
Protein and peptide mapping in biopharmaceutical analysis
Proteomics applications requiring wide-pore reversed-phase columns
Biochemical analysis of peptides, protein fragments, and glycopeptides
Method development requiring complementary selectivity to C4 and C18
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C8 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 4 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 300 C8, 3 µm | r33.8e. |
| ReproSil-Pur 300 C8, 5 µm | r35.8e. |
| ReproSil-Pur 300 C8, 10 µm | r30.8e. |
| ReproSil-Pur 300 C8, 15 µm | r315.8e. |