ReproSil-Pur 300 Si is an unbonded, high-purity silica HPLC column with a 300 Å wide-pore structure, optimized for normal-phase and adsorption chromatography of proteins, peptides, nucleic acids, and other large biomolecules. The large pore size ensures excellent accessibility to the silica surface, enabling efficient interactions even with high-molecular-weight analytes.
With its active silanol groups, ReproSil-Pur 300 Si provides strong polar retention and selectivity, while the high-purity silica minimizes secondary interactions, delivering sharp peak shapes and reproducible results. It is ideally suited for laboratories requiring robust and reliable normal-phase separations of biomolecules.
ReproSil-Pur 300 Si combines ultra-pure wide-pore silica with strong polar selectivity – the dependable choice for laboratories performing normal-phase HPLC separations of large biomolecules.
Unbonded, high-purity silica stationary phase
300 Å wide-pore structure for proteins, peptides, and nucleic acids
Strong polar interactions for normal-phase chromatography
High reproducibility and sharp peak shapes
Available particle sizes: 3 µm, 5 µm, 10 µm
Normal-phase separations of peptides, proteins, and nucleic acids
Biopharmaceutical analysis of large biomolecules and impurities
Biochemical studies requiring polar selectivity for macromolecules
Method development with orthogonal selectivity to reversed-phase HPLC
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | Si |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | No |
| Carbonload | 0 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 300 Si, 3 µm | r33.00. |
| ReproSil-Pur 300 Si, 5 µm | r35.00. |
| ReproSil-Pur 300 Si, 10 µm | r30.00. |