ReproSil-Pur 300 Phenyl

Wide-Pore Aromatic Column for π–π Selectivity in Biomolecule Analysis

ReproSil-Pur 300 Phenyl is a silica-based HPLC column with phenyl bonded chemistry on high-purity 300 Å silica. The wide-pore structure ensures excellent accessibility for proteins, peptides, and other large biomolecules, while the phenyl functionality introduces π–π interactions and complementary selectivity to standard alkyl phases.

This combination makes ReproSil-Pur 300 Phenyl particularly valuable for separating aromatic amino acids, peptide variants, and structurally similar biomolecules. Robust bonding chemistry ensures sharp peak shapes, reproducibility, and long column lifetimes even under demanding gradient conditions.

ReproSil-Pur 300 Phenyl combines wide-pore accessibility with aromatic selectivity – the dependable choice for laboratories analyzing proteins and peptides with π–π interactions in HPLC.

Key Features:

  • High-purity silica with phenyl bonded chemistry

  • 300 Å wide-pore structure for proteins, peptides, and biomolecules

  • π–π interactions for enhanced selectivity of aromatic analytes

  • Complementary selectivity to conventional C18 and C8 phases

  • Available particle sizes: 3 µm, 5 µm

Typical Applications:

  • Biopharmaceutical analysis of proteins and aromatic peptide variants

  • Proteomics applications requiring aromatic selectivity

  • Separation of aromatic amino acids and modified biomolecules

  • Method development requiring orthogonal selectivity to alkyl phases

Specifications

Request Product
Particle Sizes
  • 3 µm
  • 5 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification Phenyl
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 3 %
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil-Pur 300 Phenyl, 3 µm r33.pe.
ReproSil-Pur 300 Phenyl, 5 µm r35.pe.
ReproSil-Pur 300 ODS-3 ReproSil-Pur 300 Si

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info@dr-maisch.com +49 7073 5035 7

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