ReproSil Saphir 1000 C18-2 HE is a premium reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica featuring an exceptionally large pore size of 1000 Å. Functionalized with C18 ligands and a high degree of endcapping, this phase combines strong hydrophobic retention with excellent peak symmetry and reproducibility, even for challenging polar and basic analytes. The extremely wide pore size makes this column especially suitable for very large biomolecules such as antibodies, proteins, glycoproteins, and oligonucleotides, ensuring efficient mass transfer and optimal accessibility to the bonded ligands.
ReproSil Saphir 1000 C18-2 HE from Dr. Maisch HPLC GmbH delivers robust and reproducible wide-pore performance – the ideal choice for laboratories working with large biomolecules in advanced reversed-phase HPLC.
Ultra-wide pore size (1000 Å) spherical silica for biomacromolecules
C18 bonding with high endcapping for excellent peak shape
Strong hydrophobic retention with minimized silanol interactions
Designed for peptides, proteins, oligonucleotides, and antibodies
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 7 µm and 10 µm particle sizes
Reversed-phase HPLC of monoclonal antibodies and large proteins
Bioanalytical separations of oligonucleotides and nucleic acids
Proteomics and biopharmaceutical method development
Stability-indicating assays for therapeutic biomolecules
| Particle Sizes |
|
|---|---|
| Pore Size | 1 000 Å |
| Surface Area | 30 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 3 % |
| pH Range | 2 - 8 |
| Phase | Material Number |
|---|---|
| ReproSil Saphir 1000 C18-2 HE, 7 µm | ra67.9e. |
| ReproSil Saphir 1000 C18-2 HE, 10 µm | ra60.9e. |