ReproSil Saphir 300 CM from Dr. Maisch HPLC GmbH is a high-purity silica-based HPLC column with a 300 Å wide-pore structure and a carboxymethyl weak cation exchange bonded phase. This stationary phase offers controlled ionic interactions for positively charged analytes, making it ideal for the separation of proteins, peptides, and other biomolecules with basic functional groups. The wide-pore design ensures excellent accessibility and efficient mass transfer for high-molecular-weight analytes, while the CM functionality provides milder ion-exchange conditions compared to strong cation exchangers, preserving biomolecule structure and activity.
ReproSil Saphir 300 CM columns from Dr. Maisch HPLC GmbH combine wide-pore accessibility with gentle ion-exchange selectivity – the ideal choice for sensitive large biomolecule HPLC separations.
Fully porous, ultra-pure silica with 300 Å pore size
Weak cation exchange bonded phase for gentle protein separations
Optimized for large biomolecules including proteins and peptides
High resolution, reproducibility, and peak symmetry
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 5 µm and 10 µm particle sizes
Protein and peptide separations under mild ion-exchange conditions
Biopharmaceutical QC and characterization of basic biomolecules
Fractionation of protein isoforms and charge variants
Method development for biomolecule purification
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | Carboxymethyl |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Carbonload | 3 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil Saphir 300 CM, 5 µm | ra35.cm. |
| ReproSil Saphir 300 CM, 10 µm | ra30.cm. |