ReproSil Saphir 300 SCX

Wide-Pore Strong Cation Exchange Column for Large Biomolecule Separations

ReproSil Saphir 300 SCX from Dr. Maisch HPLC GmbH is a high-purity silica-based HPLC column with a 300 Å wide-pore structure and a strong cation exchange bonded phase based on sulfonic acid functional groups. This phase provides strong ionic retention for positively charged analytes, delivering high resolution, sharp peak shapes, and reproducible retention times. The wide-pore design ensures optimal accessibility and efficient mass transfer for high-molecular-weight analytes such as proteins, enzymes, and peptides, making it particularly suitable for biopharmaceutical and proteomics applications.

ReproSil Saphir 300 SCX columns from Dr. Maisch HPLC GmbH combine wide-pore accessibility with strong ionic selectivity – the optimal choice for high-resolution large biomolecule chromatography.

Key Features:

  • Fully porous, ultra-pure silica with 300 Å pore size

  • Strong cation exchange (SCX) phase with sulfonic acid groups

  • High selectivity for basic biomolecules and peptides

  • Excellent reproducibility, resolution, and peak symmetry

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm, 10 µm, and 15 µm particle sizes

Typical Applications:

  • Protein and peptide separations via strong cation exchange

  • Biopharmaceutical QC and charge variant analysis

  • Fractionation of protein isoforms in proteomics

  • Preparative purification of basic biomolecules

Specifications

Request Product
Particle Sizes
  • 5 µm
  • 10 µm
  • 15 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification SCX
Particle Type
Separation Mode
USP Codes
Endcapping No
Carbonload 6 %
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil Saphir 300 SCX, 5 µm ra35.scx.
ReproSil Saphir 300 SCX, 10 µm ra30.scx.
ReproSil Saphir 300 SCX, 15 µm ra315.scx.
ReproSil Saphir 300 CM

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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