ReproSil 5000 SEC

Silica-Based Column for High-Molecular-Weight Size Exclusion Chromatography

ReproSil 5000 SEC is a silica-based HPLC column designed for size exclusion chromatography (SEC) in the molecular weight range of 150,000 – 1,250,000 Da. With its large pore structure, the column is optimized for the separation and characterization of very large biomolecules and high-molecular-weight polymers. The stationary phase ensures accurate size-based fractionation with minimal secondary interactions, while providing excellent mechanical stability and reproducibility. This makes ReproSil 5000 SEC the ideal choice for analytical and preparative SEC applications in biotechnology, polymer science, and pharmaceutical research.

ReproSil 5000 SEC combines robust silica performance with large-pore SEC selectivity – the reliable choice for high-precision separations of very large biomolecules and polymers in the 150,000–1,250,000 Da range.

Key Features:

  • Silica-based stationary phase for size exclusion chromatography

  • Molecular weight separation range: 150,000 – 1,250,000 Da

  • Optimized for very large biomolecules and synthetic polymers

  • High reproducibility, robust performance, and long column lifetime

  • Suitable for analytical and preparative workflows

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm

Typical Applications:

  • Separation and analysis of very large proteins and protein complexes

  • Characterization of nucleic acids and biopolymers

  • Molecular weight distribution studies of synthetic high polymers

  • Biotechnological and pharmaceutical research involving macromolecules

Specifications

Request Product
Particle Size
  • 5 µm
Pore Size 800 Å
Surface Area 60 m²/g
Phase/Modification PEG
Particle Type
Separation Mode
USP Code
Endcapping No
Carbonload 5 %
pH Range 2 to 8

Material Number

Phase Material Number
ReproSil 5000 SEC, 5 µm r55.sec.
ReproSil 4000 SEC

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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