ReproSil 4000 SEC is a silica-based HPLC column developed for size exclusion chromatography (SEC) in the molecular weight range of 20,000 – 500,000 Da. With its optimized pore structure, the column allows efficient size-based separation of medium to large biomolecules and synthetic polymers while minimizing secondary interactions. The stationary phase provides excellent mechanical stability, reproducible retention, and sharp peak shapes, making it suitable for both analytical and preparative SEC workflows. ReproSil 4000 SEC is particularly well-suited for applications in biotechnology, pharmaceutical research, and polymer characterization.
ReproSil 4000 SEC combines optimized pore architecture with robust silica stability – the reliable choice for accurate and reproducible SEC separations in the 20,000–500,000 Da range.
Silica-based stationary phase optimized for size exclusion chromatography
Molecular weight separation range: 20,000 – 500,000 Da
Accurate size-based separation with minimal secondary interactions
High mechanical stability and reproducibility
Suitable for analytical and preparative applications
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 5 µm
Separation and characterization of proteins and enzymes
Analysis of nucleic acids and large biomolecules
Molecular weight distribution studies of biopolymers and synthetic polymers
Pharmaceutical and biochemical research involving high-molecular-weight compounds
| Particle Size |
|
|---|---|
| Pore Size | 400 Å |
| Surface Area | 120 m²/g |
| Phase/Modification | PEG |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Carbonload | 11 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil 4000 SEC, 5 µm | r45.sec. |