ReproSil-XR 300 C4

Wide-Pore C4 Column for Gentle Large Biomolecule Separations

ReproSil-XR 300 C4 from Dr. Maisch HPLC GmbH is a high-purity silica-based wide-pore HPLC column with a 300 Å pore size and a butyl bonded phase. The short alkyl chain provides lower hydrophobic retention than C18 or C8, allowing milder elution conditions that help preserve the structure and bioactivity of proteins, peptides, and other sensitive large biomolecules. The wide-pore design ensures excellent accessibility and efficient mass transfer, making this column ideal for analytical and preparative separations in biopharmaceutical and proteomics workflows.

ReproSil-XR 300 C4 columns from Dr. Maisch HPLC GmbH combine wide-pore accessibility with gentle separation conditions – the ideal choice for high-performance biomolecule chromatography.

Key Features:

  • Fully porous, ultra-pure silica with 300 Å pore size

  • C4 bonding – USP L26 classification

  • Lower hydrophobic retention for gentle biomolecule analysis

  • Excellent reproducibility, resolution, and peak symmetry

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 3 µm, 5 µm, and 10 µm particle sizes

Typical Applications:

  • Protein and peptide separations under mild conditions

  • Biopharmaceutical QC and characterization of therapeutic proteins

  • Gradient HPLC of hydrophobic biomolecules

  • Preparative purification of sensitive analytes

Specifications

Request Product
Particle Sizes
  • 3 µm
  • 5 µm
  • 10 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification C4
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 3 %
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil-XR 300 C4, 3 µm rx33.4e.
ReproSil-XR 300 C4, 5 µm rx35.4e.
ReproSil-XR 300 C4, 10 µm rx30.4e.
ReproSil-XR 300 C8

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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