ReproSil 200 C4-Aqua

Wide-Pore Aqueous-Compatible C4 Column for Biomolecule Separations

ReproSil 200 C4-Aqua from Dr. Maisch HPLC GmbH is a fully porous, high-purity silica HPLC column with a 200 Å pore size and an aqueous-compatible butyl bonded phase. The shorter alkyl chain offers reduced hydrophobic retention compared to C18 or C8 phases, enabling gentler elution for proteins, peptides, and other biomolecules while preserving their structural integrity. Engineered to prevent phase collapse in 100% aqueous mobile phases, this column ensures stable retention, excellent peak symmetry, and high reproducibility for hydrophilic and amphiphilic biomolecules.

ReproSil 200 C4-Aqua columns from Dr. Maisch HPLC GmbH combine gentle C4 selectivity with aqueous stability – the ideal choice for sensitive protein and peptide chromatography in HPLC.

Key Features:

  • Fully porous, ultra-pure silica with 200 Å pore size

  • Aqueous-compatible C4 bonding – USP L26 classification

  • Lower hydrophobic retention for biomolecule-friendly separations

  • Stable in 100% aqueous mobile phases

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm

Typical Applications:

  • Protein and peptide separations under mild elution conditions

  • LC-MS compatible aqueous reversed-phase workflows

  • Biopharmaceutical QC and proteomics research

  • Method development for hydrophilic biomolecules

Specifications

Request Product
Particle Size
  • 5 µm
Pore Size 200 Å
Surface Area 150 m²/g
Phase/Modification C4
Particle Type
Separation Mode
USP Codes
Endcapping Yes
pH Range 2 to 8

Material Number

Phase Material Number
ReproSil 200 C4-Aqua, 5 µm r25.4aq.
ReproSil 200 C8-Aqua

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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