ReproSil 300 C4 from Dr. Maisch HPLC GmbH is a fully porous, high-purity silica HPLC column with a 300 Å pore size and a butyl bonded phase. The shorter alkyl chain provides reduced hydrophobic retention compared to C18 or C8 phases, enabling gentle elution conditions that help preserve the structural integrity of large proteins, enzymes, and biomolecules. The wide-pore structure ensures efficient mass transfer for high-molecular-weight analytes, while delivering sharp peak shapes and high reproducibility.
ReproSil 300 C4 columns from Dr. Maisch HPLC GmbH deliver gentle, reproducible separations – the ideal choice for large biomolecule chromatography in reversed-phase HPLC.
Fully porous, ultra-pure silica with 300 Å pore size
C4 bonding – USP L26 classification
Lower hydrophobic retention for mild protein separations
Optimized for large proteins, enzymes, and biomolecules
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 5 µm and 10 µm particle sizes
Protein and enzyme separations under gentle elution conditions
Peptide and biomolecule chromatography in proteomics
Biopharmaceutical QC and large-molecule characterization
Method development for sensitive high-mass analytes
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil 300 C4, 5 µm | r35.46. |
| ReproSil 300 C4, 10 µm | r30.46. |