ReproSil 300 C8

Wide-Pore C8 Column for Balanced Retention of Large Biomolecules

ReproSil 300 C8 from Dr. Maisch HPLC GmbH is a fully porous, high-purity silica HPLC column with a 300 Å pore size and an octyl bonded phase. Offering moderate hydrophobic retention, this phase is ideal for proteins, peptides, and other large biomolecules, providing faster elution than C18 phases while maintaining excellent resolution. The wide-pore design ensures efficient mass transfer for high-molecular-weight analytes, while the C8 bonding delivers sharp peak shapes, high reproducibility, and robust performance under both isocratic and gradient conditions.

ReproSil 300 C8 columns from Dr. Maisch HPLC GmbH combine balanced retention with wide-pore accessibility – the versatile choice for large biomolecule chromatography in HPLC.

Key Features:

  • Fully porous, ultra-pure silica with 300 Å pore size

  • C8 bonding – USP L7 classification

  • Balanced retention for proteins, peptides, and biomolecules

  • Faster analysis times compared to C18 wide-pore phases

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm and 10 µm particle sizes

Typical Applications:

  • Protein and peptide separations in proteomics and biopharma

  • Large biomolecule analysis in QC and R&D

  • Gradient separations of amphiphilic biomolecules

  • Method development requiring moderate hydrophobicity

Specifications

Request Product
Particle Sizes
  • 5 µm
  • 10 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification C8
Particle Type
Separation Mode
USP Code
Endcapping Yes
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil 300 C8, 5 µm r35.86.
ReproSil 300 C8, 10 µm r30.86.
ReproSil 300 C4 ReproSil 300 C18

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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