ReproSil 300 DEAE from Dr. Maisch HPLC GmbH is a fully porous, high-purity silica HPLC column with a 300 Å pore size and a diethylaminoethyl (DEAE) functionalized bonded phase. As a weak anion exchanger, this phase provides selective retention for negatively charged biomolecules such as nucleic acids, proteins, and glycoproteins under mild chromatographic conditions. The wide-pore structure ensures optimal accessibility and mass transfer for large molecules, while the DEAE chemistry enables tunable selectivity through pH and ionic strength adjustments.
ReproSil 300 DEAE columns from Dr. Maisch HPLC GmbH deliver reproducible and selective wide-pore ion exchange performance – the ideal choice for large biomolecule chromatography in HPLC.
Fully porous, ultra-pure silica with 300 Å pore size
DEAE weak anion exchange functionality
Optimized for large biomolecule accessibility and resolution
Tunable retention via mobile phase pH and salt concentration
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 5 µm
Purification and analysis of nucleic acids
Protein and glycoprotein separations in biopharma workflows
Ion-exchange method development for large biomolecules
Fractionation of negatively charged macromolecules
| Particle Size |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | DEAE |
| Particle Type | |
| Separation Modes | |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil 300 DEAE, 5 µm | r35.deae. |