Reprospher 100 NH₂ is a polar HPLC column from Dr. Maisch HPLC GmbH, based on high-purity, fully porous silica with a 100 Å pore size and bonded with aminopropyl (NH₂) groups. This phase supports multiple retention mechanisms, including normal-phase chromatography, HILIC (hydrophilic interaction liquid chromatography), and weak anion exchange. The mild anion-exchange character of the NH₂ group makes this column particularly suited for separating carbohydrates, sugar alcohols, organic acids, and other polar compounds. Thanks to its balanced selectivity and low silanol activity, Reprospher 100 NH₂ offers reproducible retention, stable peak shapes, and excellent compatibility with aqueous and organic-rich eluents.
Reprospher 100 NH₂ from Dr. Maisch HPLC GmbH combines versatile polar selectivity with robust silica performance – ideal for efficient and reliable separation of hydrophilic and ionizable analytes in HPLC.
Aminopropyl (NH₂) bonded phase – USP L8 classification
Fully porous silica with 100 Å pore size
Supports normal-phase, HILIC, and weak anion-exchange mechanisms
Stable performance with both aqueous and non-polar solvents
Available in 3 µm and 5 µm particle sizes
Separation of sugars, oligosaccharides, and polyols
Organic acids and polar drug substances
HILIC and normal-phase method development
Sample clean-up or dry loading in preparative workflows
| Particle Sizes |
|
|---|---|
| Pore Size | 100 Å |
| Surface Area | 350 m²/g |
| Phase/Modification | NH2 |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | No |
| Carbonload | 4 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 100 NH2, 3 µm | rs13.a0. |
| Reprospher 100 NH2, 5 µm | rs15.a0. |