Reprospher 200 C18-TN is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (200 Å pore size) modified with trifunctionally bonded octadecyl ligands and deliberately not endcapped. This design combines strong hydrophobic retention from the C18 chains with residual silanol activity, enabling additional polar and ionic interactions. The wide pore size makes this stationary phase especially suitable for larger analytes such as peptides, proteins, and oligonucleotides, while the absence of endcapping provides unique mixed-mode retention characteristics, valuable for method development and analytes requiring silanol interaction.
Reprospher 200 C18-TN from Dr. Maisch HPLC GmbH delivers versatile reversed-phase performance with controlled silanol activity – the ideal choice for laboratories seeking mixed-mode selectivity and robust retention of biomolecules.
Trifunctional C18 bonding without endcapping – USP L1
200 Å pore size optimized for biomacromolecules
Strong hydrophobic retention with additional silanol activity
Mixed-mode selectivity combining hydrophobic and polar interactions
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available from 3 µm to 10 µm particle sizes
Separation of peptides, proteins, and oligonucleotides
Mixed-mode retention for polar and basic analytes
Method development requiring alternative selectivity to endcapped phases
Gradient and isocratic reversed-phase separations with extended retention options
| Particle Sizes |
|
|---|---|
| Pore Size | 200 Å |
| Surface Area | 160 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Carbonload | 11 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 200 C18-TN, 3 µm | rs23.9tn. |
| Reprospher 200 C18-TN, 5 µm | rs25.9tn. |
| Reprospher 200 C18-TN, 7 µm | rs27.9tn. |
| Reprospher 200 C18-TN, 10 µm | rs20.9tn. |