Reprospher 200 C8 is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with high-purity, fully porous silica and bonded with octyl ligands. With a 200 Å pore size, this phase is optimized for the separation of peptides, small proteins, and hydrophobic compounds with moderate molecular weight. Its shorter alkyl chain offers reduced retention compared to C18, allowing faster elution of strongly hydrophobic analytes while maintaining excellent resolution and peak symmetry. The wide-pore structure ensures effective mass transfer and accessibility for larger analytes, making it a versatile choice for bioanalytical and pharmaceutical reversed-phase applications.
This wide-pore octyl phase from Dr. Maisch HPLC GmbH delivers balanced retention and high performance – ideal for reversed-phase separations involving hydrophobic and medium-sized biomolecules.
Octyl (C8) bonded reversed-phase phase – USP L7 classification
Fully porous silica with 200 Å pore size for peptides and hydrophobic analytes
Moderate hydrophobicity for controlled retention behavior
Excellent peak shape and reproducibility under acidic to neutral pH
Available in 3 µm and 5 µm particle sizes
Peptides, protein fragments, and lipophilic drug substances
Faster elution of strongly retained compounds
Method development for mid-sized biomolecules
Routine LC-MS and reversed-phase workflows in life sciences
| Particle Sizes |
|
|---|---|
| Pore Size | 200 Å |
| Surface Area | 160 m²/g |
| Phase/Modification | C8 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 200 C8, 3 µm | rs23.8e. |
| Reprospher 200 C8, 5 µm | rs25.8e. |