Reprospher 200 C18 is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, based on high-purity, fully porous silica with a 200 Å pore size and bonded with octadecyl groups. The wide-pore structure allows improved mass transfer and accessibility for large biomolecules such as peptides, small proteins, and hydrophobic macromolecules. Its strong hydrophobic retention and excellent peak symmetry make it a powerful tool for bioanalytical and pharmaceutical applications. This phase combines the benefits of classical reversed-phase selectivity with optimized porosity for high-molecular-weight compounds.
With its optimized pore structure and strong hydrophobic interaction, this wide-pore C18 phase from Dr. Maisch HPLC GmbH is ideal for efficient reversed-phase separations of biomolecular compounds.
Octadecylsilane (C18) bonded phase – USP L1 classification
Wide-pore silica with 200 Å pore size for large molecules
Excellent retention and peak shape for peptides and hydrophobic biomolecules
Fully porous, high-purity silica support
Available in 2.5 µm, 3 µm, and 5 µm particle sizes
Peptide mapping and peptide-based APIs
Separation of oligopeptides, protein fragments, and lipophilic biomolecules
Bioanalytical reversed-phase LC-MS
Method development for mid-size to large analytes
| Particle Sizes |
|
|---|---|
| Pore Size | 200 Å |
| Surface Area | 160 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 9 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 200 C18, 2.5 µm | rs225.9e. |
| Reprospher 200 C18, 3 µm | rs23.9e. |
| Reprospher 200 C18, 5 µm | rs25.9e. |