Reprospher 300 C4 is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with high-purity, fully porous silica featuring a 300 Å pore size and bonded with butyl ligands. The short alkyl chain offers lower hydrophobic retention compared to C18 or C8 phases, making it particularly suitable for large, hydrophobic biomolecules that elute too slowly or tail on longer-chain columns. Its wide-pore structure ensures efficient interaction with proteins, peptides, and other high-molecular-weight analytes, while maintaining excellent peak shape and reproducibility in acidic to neutral conditions.
This wide-pore C4 phase from Dr. Maisch HPLC GmbH is ideal for high-resolution separations of large, lipophilic analytes where shorter retention and excellent peak shape are required.
Butyl (C4) bonded reversed-phase phase – USP L26 classification
Fully porous silica with 300 Å pore size for large biomolecules
Lower hydrophobicity for faster elution of strongly retained analytes
High efficiency and sharp peak symmetry for peptides and proteins
Available in 1.8 µm, 2 µm, 3 µm and 5 µm particle sizes
Intact proteins, membrane proteins, and hydrophobic peptides
Reversed-phase LC-MS of large biomolecules
Method development requiring reduced retention strength
Bioanalytical workflows under acidic pH
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 2.5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C4, 1.8 µm | rs318.4e. |
| Reprospher 300 C4, 2 µm | rs32.4e. |
| Reprospher 300 C4, 3 µm | rs33.4e. |
| Reprospher 300 C4, 5 µm | rs35.4e. |