Reprospher 300 C4-Aqua is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, based on fully porous, high-purity silica with a 300 Å pore size and bonded with butyl ligands plus a polar surface modification. This Aqua-compatible phase ensures stable performance in 100% aqueous mobile phases and improves retention of polar peptides, glycopeptides, and hydrophilic proteins. Thanks to its short alkyl chain and wide-pore structure, the column enables efficient mass transfer, sharp peak shapes, and reproducible retention of large, water-soluble biomolecules—particularly in LC-MS workflows.
This wide-pore, Aqua-stabilized phase from Dr. Maisch HPLC GmbH combines C4 selectivity with aqueous compatibility – ideal for high-resolution reversed-phase separations of polar and large biomolecular analytes.
Butyl (C4) bonded phase with Aqua modification for 100% aqueous operation
Fully porous silica with 300 Å pore size for large polar biomolecules
Enhanced retention of hydrophilic peptides and proteins
Ideal for reversed-phase LC-MS using water-rich eluents
Available in 1.8 µm and 5 µm particle sizes
Polar peptides, glycoproteins, and hydrophilic biotherapeutics
Aqueous-compatible reversed-phase separations under low-organic conditions
Bioanalytical method development with LC-MS detection
Early-eluting biomolecules requiring enhanced retention
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Modes | |
| USP Codes | |
| Endcapping | Yes |
| Carbonload | 3 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C4-Aqua, 1.8 µm | rs318.4aq. |
| Reprospher 300 C4-Aqua, 5 µm | rs35.4aq. |