Reprospher 300 Phenyl-DE is a high-performance reversed-phase HPLC column from Dr. Maisch HPLC GmbH, based on ultra-pure, fully porous spherical silica (300 Å pore size) functionalized with phenyl groups and additionally double endcapped to suppress residual silanol activity. This unique bonding provides both hydrophobic retention and π–π interaction selectivity, making it particularly effective for the separation of aromatic compounds, isomers, peptides, and proteins. The wide 300 Å pore size allows efficient mass transfer and accessibility for large biomolecules, while the double endcapping ensures excellent peak symmetry, reproducibility, and stability, even for challenging basic analytes.
Reprospher 300 Phenyl-DE from Dr. Maisch HPLC GmbH offers robust aromatic selectivity with superior peak symmetry – the ideal column for laboratories analyzing biomolecules and aromatic analytes in advanced reversed-phase HPLC.
Phenyl-bonded silica with double endcapping – USP L11
300 Å pore size for proteins, peptides, and large biomolecules
Dual retention mechanism: hydrophobic + π–π interactions
Excellent peak shape and reproducibility for aromatic analytes
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 3 µm
Separation of aromatic pharmaceuticals and structural isomers
Reversed-phase HPLC of peptides and proteins with aromatic residues
Bioanalytical workflows requiring enhanced π–π selectivity
Gradient and isocratic separations of complex biomolecules
| Particle Size |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | Phenyl |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 Phenyl-DE, 3 µm | rs33.pde. |