Reprospher 300 Phenyl is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with fully porous, high-purity silica featuring a 300 Å pore size and bonded with phenyl ligands. The aromatic surface enables π–π and dipole interactions in addition to hydrophobic retention, providing orthogonal selectivity to conventional C18 and C8 phases. Its wide-pore structure supports the separation of large, aromatic, and structurally related biomolecules such as peptides, protein domains, and bioactive compounds — especially when resolution between isomers or planar structures is required.
This wide-pore phenyl phase from Dr. Maisch HPLC GmbH enables selective, high-resolution separations for complex, π-rich biomolecules — ideal for challenging reversed-phase applications.
Phenyl bonded reversed-phase phase – USP L11 classification
Fully porous silica with 300 Å pore size for high-molecular-weight analytes
π–π and polar selectivity for aromatic and planar compounds
Excellent peak shape and retention stability under acidic conditions
Available in 10 µm
Aromatic peptides, protein fragments, and glycopeptides
Isomeric or structurally similar biomolecules
Orthogonal method development to C18-based phases
LC-MS and UV detection in pharmaceutical and bioanalytical workflows
| Particle Size |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | Phenyl |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 4 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 Phenyl, 10 µm | rs30.pe. |