Ultrasep ES 100 RP4 is a silica-based HPLC column with a 100 Å pore size and a C4 (butyl) bonded phase. The short alkyl chains provide moderate hydrophobic retention, allowing faster elution compared to C8 or C18 phases while still retaining non-polar analytes more strongly than very short-chain phases such as C1 or C3.
This column delivers robust performance, sharp peak shapes, and high reproducibility, making it particularly suitable for method development, peptide analysis, and applications where reduced retention and faster run times are advantageous.
Ultrasep ES 100 RP4 combines moderate retention, reproducible performance, and shorter analysis times – the versatile choice for efficient reversed-phase HPLC separations.
Silica-based stationary phase with 100 Å pore size
Butyl (C4) bonded phase – USP L26 classification
Moderate hydrophobic retention with faster elution than C8/C18
High reproducibility and excellent peak symmetry
Robust performance for routine and advanced analyses
Available particle sizes: 5 µm, 7 µm, 10 µm
Peptide and small protein analysis with reduced retention
Pharmaceutical impurity profiling requiring shorter run times
Environmental and food analysis of moderately hydrophobic compounds
Method development with alternative short-chain selectivity
| Particle Sizes |
|
|---|---|
| Pore Size | 100 Å |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| pH Range | 2 - 8 |
| Phase | Material Number |
|---|---|
| Ultrasep ES 100 RP4, 5 µm | u15.40. |
| Ultrasep ES 100 RP4, 7 µm | u17.40. |
| Ultrasep ES 100 RP4, 10 µm | u10.40. |