ReproSil Chiral-L-Leucin

Silica-Based Brush-Type Chiral Phase with N-(3,5-dinitrobenzoyl)-L-leucine for Enantioselective Chromatography

ReproSil Chiral-L-Leucin is a silica-based HPLC stationary phase of the brush-type Pirkle design, functionalized with N-(3,5-dinitrobenzoyl)-L-leucine as the chiral selector. This phase is specifically developed for the enantioselective separation of benzodiazepines and related aromatic compounds, offering strong chiral discrimination through a combination of hydrogen bonding, π–π interactions, dipole–dipole forces, and steric complementarity.

The L-leucine moiety provides a defined chiral environment, while the electron-withdrawing 3,5-dinitrobenzoyl group introduces π–acceptor functionality that enhances interactions with aromatic and heteroaromatic analytes. This synergistic structure results in highly selective and efficient enantioseparations, particularly for compounds containing π–electron-rich systems and hydrogen bond donor groups — characteristics typical of benzodiazepines and pharmaceutical intermediates. The chiral selector is covalently bonded to high-purity spherical silica, ensuring excellent mechanical stability, solvent compatibility, and long operational lifetime. The phase can be used in normal-phase, reversed-phase, or polar-organic chromatographic modes, providing flexibility for analytical method development and routine enantioselective analysis in pharmaceutical, biochemical, and forensic applications.

ReproSil Chiral-L-Leucin combines the chiral recognition ability of L-leucine with the strong π–acceptor characteristics of the N-(3,5-dinitrobenzoyl) group – the reliable choice for precise, high-resolution, and reproducible enantioselective separations of benzodiazepines and other aromatic chiral compounds.