ReproSil Chiral-L-Prolin

Silica-Based Chiral Phase Modified with L-Proline for Enantioselective Chromatography

ReproSil Chiral-L-Prolin is a silica-based HPLC stationary phase modified with the natural amino acid L-proline, developed for enantioselective separations based on chiral ion-exchange and ligand-exchange mechanisms. The immobilized L-proline functionality provides both a chiral environment and reactive sites capable of forming transient diastereomeric complexes with analytes containing carbonyl, hydroxyl, or amino groups near the stereogenic center.

Through a combination of acid–base interactions, hydrogen bonding, and metal-mediated coordination, the phase enables highly selective separations of amino acids, peptides, α-hydroxy acids, and other polar chiral compounds. The well-defined stereochemistry of L-proline contributes to reliable enantiomer discrimination and reproducible retention behavior. The chiral selector is covalently attached to high-purity spherical silica, providing excellent mechanical stability, chemical robustness, and compatibility with aqueous or mixed organic mobile phases. ReproSil Chiral-L-Prolin is ideal for analytical and preparative use in biochemical, pharmaceutical, and environmental laboratories, where gentle yet precise chiral recognition is required.

ReproSil Chiral-L-Prolin utilizes the intrinsic chirality and coordinating ability of L-proline to deliver precise and reproducible enantioselective separations – the dependable choice for the resolution of polar and biologically relevant chiral compounds.