ReproSil Chiral-MID

Immobilized Amylose-Based Chiral Phase with Tris-(3-chlorophenyl)carbamate for Enantioselective Normal-Phase Chromatography

ReproSil Chiral-MID is a silica-based HPLC stationary phase with immobilized tris-(3-chlorophenyl)carbamate amylose, specifically developed for enantioselective separations under normal-phase conditions. The immobilized amylose derivative substituted with 3-chlorophenyl carbamate groups provides strong and versatile chiral recognition through hydrogen bonding, π–π stacking, dipole–dipole interactions, and steric complementarity, enabling high-resolution separations of a broad range of racemic compounds.

The chlorine substituent on the aromatic ring introduces a subtle electronic effect that fine-tunes the polarity and π-acceptor properties of the chiral selector, resulting in distinct and complementary selectivity for various compound classes. The covalent immobilization of the chiral selector ensures exceptional chemical and mechanical stability, allowing the use of a wide variety of mobile phases — including polar and mixed solvent systems — without loss of chiral performance. The stationary phase is built on high-purity spherical silica, providing excellent efficiency, reproducibility, and long column lifetime. ReproSil Chiral-MID is ideally suited for pharmaceutical, chemical, and biochemical applications, particularly for analytical and preparative enantioseparations where high robustness and solvent flexibility are essential.

ReproSil Chiral-MID combines the proven chiral selectivity of amylose tris-(3-chlorophenyl)carbamate with the enhanced stability of an immobilized silica support – the reliable choice for accurate, high-resolution, and reproducible enantioselective separations in normal-phase chromatography.

Attractive alternative to CHIRALPAK® ID (Daicel®).

For the use of our ReproSil Chiral-MID columns, please refer to our Column Care Guide.

Key Features:

  • Silica-based stationary phase with immobilized tris-(3-chlorophenyl)carbamate amylose

  • Designed for enantioselective separations under normal-phase conditions

  • Strong chiral recognition via hydrogen bonding, π–π stacking, and dipole–dipole interactions

  • Distinct selectivity influenced by chlorine substituents on the aromatic rings

  • Exceptional chemical stability and extended solvent compatibility

  • High mechanical strength and long operational lifetime

  • Suitable for analytical and preparative chiral HPLC applications

Typical Applications:

  • Enantioselective separation of pharmaceuticals, fine chemicals, and intermediates

  • Resolution of racemic mixtures containing aromatic or halogenated structures

  • Method development in normal-phase and polar-organic chiral HPLC

  • Stereochemical purity testing and quality control in pharmaceutical and chemical research

Specifications

Request Product
Particle Size
  • 5 µm
Pore Size 1 000 Å
Surface Area 30 m²/g
Phase/Modification Tris-(3-chlorophenyl)carbamat-Amylose
Particle Type
Separation Mode
Endcapping No
pH Range 2 to 8

Material Number

Phase Material Number
ReproSil Chiral-MID, 5 µm r65.mid.
ReproSil Chiral-MIC ReproSil Chiral-MIF

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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