ReproSil Chiral-MIF is a silica-based HPLC stationary phase with immobilized tris-(3-chloro-4-methylphenyl)carbamate amylose, developed for enantioselective separations under normal-phase conditions. The amylose derivative, functionalized with 3-chloro-4-methylphenyl carbamate groups, provides a finely tuned balance of electronic and steric effects, enabling highly efficient chiral recognition for a broad range of racemic analytes.
Chiral discrimination on ReproSil Chiral-MIF is achieved through multiple interaction mechanisms, including hydrogen bonding, π–π stacking, dipole–dipole interactions, and steric complementarity between the analyte and the chiral selector. The presence of chlorine and methyl substituents on the aromatic rings adjusts both the polarity and the electronic distribution of the carbamate moieties, resulting in a distinct selectivity profile and enhanced resolution for structurally diverse compounds.
Through covalent immobilization of the amylose derivative onto high-purity spherical silica, the phase offers exceptional chemical and mechanical stability while maintaining excellent enantioselectivity and reproducibility. This immobilized design allows the use of a wide range of solvents, including more polar or unconventional mobile phases, without compromising column performance. ReproSil Chiral-MIF is ideally suited for pharmaceutical, biochemical, and fine chemical applications, providing robust, reproducible, and high-resolution chiral separations for both method development and routine analysis.
ReproSil Chiral-MIF combines the chiral recognition strength of amylose tris-(3-chloro-4-methylphenyl)carbamate with the robustness of immobilized silica technology – the reliable choice for accurate, reproducible, and high-resolution enantioselective separations in normal-phase chromatography.
Attractive alternative to CHIRALPAK® IF (Daicel®).
For the use of our ReproSil Chiral-MIF columns, please refer to our Column Care Guide.
Silica-based stationary phase with immobilized tris-(3-chloro-4-methylphenyl)carbamate amylose
Designed for enantioselective separations under normal-phase conditions
Strong chiral recognition through hydrogen bonding, π–π, and dipole–dipole interactions
Distinct selectivity due to electronic and steric effects of chloro and methyl substituents
Covalently immobilized coating for excellent chemical stability and solvent flexibility
High mechanical strength and reproducible performance
Suitable for analytical and preparative chiral HPLC applications
Enantioselective separation of pharmaceuticals, fine chemicals, and intermediates
Analysis of racemic compounds containing aromatic or halogenated groups
Method development using flexible solvent systems in normal-phase HPLC
Quality control and stereochemical purity testing in pharmaceutical and chemical research
| Particle Sizes |
|
|---|---|
| Pore Size | 1 000 Å |
| Surface Area | 30 m²/g |
| Phase/Modification | Tris-(3-chloro-4-methylphenyl)carbamat-Amylose |
| Particle Type | |
| Separation Modes | |
| Endcapping | No |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil Chiral-MIF, 5 µm | r65.mif. |
| ReproSil Chiral-MIF, 10 µm | r60.mif. |