ReproSil Chiral-MIX

Immobilized Cellulose-Based Chiral Phase with Tris-(4-chloro-3-methylphenyl)carbamate for Enantioselective Normal-Phase Chromatography

ReproSil Chiral-MIX is a silica-based HPLC stationary phase with immobilized tris-(4-chloro-3-methylphenyl)carbamate cellulose, developed for enantioselective separations under normal-phase conditions. The cellulose derivative substituted with 4-chloro-3-methylphenyl carbamate groups provides highly efficient and versatile chiral recognition through a combination of hydrogen bonding, π–π stacking, dipole–dipole interactions, and steric complementarity, enabling precise and reproducible separations of a wide range of racemic analytes.

The specific substitution pattern of chlorine and methyl groups on the aromatic rings modifies both the electronic and steric environment of the carbamate moieties, resulting in a distinct selectivity profile that allows the resolution of structurally diverse chiral compounds. The immobilization of the cellulose derivative onto high-purity spherical silica ensures exceptional chemical and mechanical stability, allowing the use of polar, non-standard, or mixed mobile phases without loss of enantioselectivity. ReproSil Chiral-MIX combines the proven chiral recognition properties of cellulose-based stationary phases with the robustness and flexibility of immobilized silica technology. It is ideally suited for pharmaceutical, chemical, and biochemical laboratories, offering high-performance enantioselective separations for analytical, semi-preparative, and preparative applications.

ReproSil Chiral-MIX combines the refined chiral recognition of cellulose tris-(4-chloro-3-methylphenyl)carbamate with the enhanced robustness of immobilized silica – the reliable choice for accurate, flexible, and reproducible enantioselective separations in normal-phase chromatography.

For the use of our ReproSil Chiral-MIX columns, please refer to our Column Care Guide.

Key Features:

  • Silica-based stationary phase with immobilized tris-(4-chloro-3-methylphenyl)carbamate cellulose

  • Designed for enantioselective separations under normal-phase conditions

  • Strong chiral recognition via hydrogen bonding, π–π stacking, and dipole–dipole interactions

  • Distinct electronic and steric properties due to chlorine and methyl substituents

  • Exceptional chemical and mechanical stability for long column lifetime

  • Extended solvent compatibility for flexible method development

  • Suitable for analytical and preparative chiral HPLC applications

Typical Applications:

  • Enantioselective separation of pharmaceuticals, fine chemicals, and intermediates

  • Resolution of racemic compounds containing aromatic or halogenated functional groups

  • Method development and chiral screening under normal-phase conditions

  • Stereochemical purity testing and quality control in pharmaceutical and chemical research

Specifications

Request Product
Particle Sizes
  • 5 µm
  • 10 µm
Pore Size 1 000 Å
Surface Area 30 m²/g
Phase/Modification Tris-(4-chloro-3-methylphenyl)carbamat-Cellulose
Particle Type
Separation Modes
Endcapping No
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil Chiral-MIX, 5 µm r65.mix.
ReproSil Chiral-MIX, 10 µm r60.mix.
ReproSil Chiral-MIF ReproSil Chiral-MIZ

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