ReproSil Chiral-TAG

Silica-Based Chiral Phase with Immobilized Teicoplanin Aglycone for Enantioselective Chromatography

ReproSil Chiral-TAG is a silica-based HPLC stationary phase with immobilized teicoplanin aglycone, a glycopeptide derivative that retains the core chiral recognition sites of teicoplanin while offering a more hydrophobic surface and distinct selectivity profile. This immobilized phase is designed for enantioselective separations of a broad range of acidic, basic, and neutral compounds, providing excellent resolution and reproducibility under various chromatographic conditions.

The teicoplanin aglycone selector forms a complex chiral recognition environment combining hydrogen bonding, π–π interactions, dipole–dipole forces, ionic interactions, and hydrophobic effects. The absence of sugar moieties in the aglycone structure increases hydrophobicity and alters the balance of polar and nonpolar interactions, allowing complementary selectivity to teicoplanin-based chiral phases. This makes ReproSil Chiral-TAG particularly useful for analytes that show weak or overlapping separations on other glycopeptide-based columns.

The teicoplanin aglycone is covalently immobilized onto high-purity spherical silica, providing exceptional chemical and mechanical stability and preventing selector leaching. The immobilized design ensures compatibility with a wide range of mobile phases, including normal-phase, reversed-phase, polar-organic, and aqueous systems, making the phase versatile for method development and routine use. ReproSil Chiral-TAG delivers robust, high-efficiency enantioseparations for a wide variety of compound classes and is ideally suited for pharmaceutical, biochemical, and clinical laboratories, where reliable chiral performance and reproducibility are essential.

ReproSil Chiral-TAG combines the robust chiral recognition of immobilized teicoplanin aglycone with the mechanical strength of high-purity silica – the reliable choice for precise, reproducible, and versatile enantioselective separations across a broad range of compound classes and chromatographic conditions.

Key Features:

  • Silica-based stationary phase with immobilized teicoplanin aglycone

  • Broad chiral selectivity for acidic, basic, and neutral compounds

  • Multiple interaction mechanisms: hydrogen bonding, ionic, π–π, dipole–dipole, and hydrophobic interactions

  • Distinct selectivity profile due to increased hydrophobicity and absence of glycosidic residues

  • Exceptional chemical and mechanical stability with extended solvent compatibility

  • Operates in normal-phase, reversed-phase, polar-organic, and aqueous modes

  • Suitable for analytical and preparative chiral HPLC applications

Typical Applications:

  • Enantioselective separation of acidic, basic, and zwitterionic pharmaceuticals

  • Resolution of amino acids, peptides, and bioactive chiral compounds

  • Analysis of hydrophobic analytes and compounds poorly resolved on teicoplanin phases

  • Method development and optimization in pharmaceutical and biochemical research

  • Quality control and stereochemical purity testing in life science and pharmaceutical laboratories

Specifications

Request Product
Particle Sizes
  • 5 µm
  • 8 µm
  • 10 µm
Pore Size 100 Å
Surface Area 300 m²/g
Phase/Modification Teicoplanin Aglycon
Particle Type
Separation Mode
USP Code
Endcapping No
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil Chiral-TAG, 5 µm r15.tag.
ReproSil Chiral-TAG, 8 µm r18.tag.
ReproSil Chiral-TAG, 10 µm r10.tag.
ReproSil Chiral-PS ReproSil Chiral-TG

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info@dr-maisch.com +49 7073 5035 7

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