ReproSil-Pur 120 C4

Short-Chain Alkyl HPLC Column for Medium Hydrophobicity

ReproSil-Pur 120 C4 is a silica-based HPLC column with butyl bonded chemistry on high-purity 120 Å silica. With shorter alkyl chains than C8 or C18 phases, it provides moderate hydrophobic retention, making it well suited for analytes that require reduced retention times or complementary selectivity in method development.

The 120 Å pore size is optimized for small to medium-sized molecules, while the robust bonding chemistry ensures reproducibility, sharp peak shapes, and long operational lifetimes. ReproSil-Pur 120 C4 is a versatile option for pharmaceutical, biochemical, and environmental laboratories.

ReproSil-Pur 120 C4 provides balanced hydrophobic retention and robust performance – the dependable choice for laboratories requiring flexible reversed-phase HPLC separations.

Key Features:

  • High-purity silica with butyl (C4) bonded chemistry

  • 120 Å pore size for small to medium-sized molecules

  • Moderate hydrophobic retention with faster elution than C8/C18

  • Robust bonding ensures reproducibility and long column lifetime

  • Available particle sizes: 3 µm - 20 µm

Typical Applications:

  • Pharmaceutical impurity profiling and stability-indicating assays

  • Biochemical separations of moderately hydrophobic drugs and metabolites

  • Food and beverage analysis of additives, preservatives, and contaminants

  • Environmental monitoring of organic pollutants

  • Method development requiring complementary selectivity to longer alkyl phases

Specifications

Request Product
Particle Sizes
  • 3 µm
  • 5 µm
  • 10 µm
  • 20 µm
Pore Size 120 Å
Surface Area 300 m²/g
Phase/Modification C4
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 7 %
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil-Pur 120 C4, 3 µm r13.4e.
ReproSil-Pur 120 C4, 5 µm r15.4e.
ReproSil-Pur 120 C4, 10 µm r10.4e.
ReproSil-Pur 120 C4, 20 µm r120.4e.
ReproSil-Pur 120 C1 ReproSil-Pur 120 C8

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