ReproSil-Pur 200 Diol is a silica-based HPLC column with bonded diol functionality on high-purity 200 Å silica. The wide-pore structure provides excellent accessibility for biomolecules such as peptides, glycopeptides, and oligosaccharides, while the diol surface offers controlled hydrophilicity for hydrophilic interaction chromatography (HILIC) and size-based separations.
Compared to bare silica, the bonded diol groups reduce strong silanol activity, ensuring improved reproducibility and peak symmetry, especially for polar and biomolecular analytes. This makes ReproSil-Pur 200 Diol a versatile tool for biopharmaceutical, biochemical, and food analysis.
ReproSil-Pur 200 Diol combines wide-pore accessibility with controlled hydrophilicity – the reliable choice for laboratories analyzing peptides, oligosaccharides, and other polar biomolecules.
High-purity silica with bonded diol functionality
200 Å wide-pore structure for peptides and medium biomolecules
Suitable for HILIC and size-based separations
Reduced silanol activity for better peak symmetry of polar compounds
Available particle sizes: 1.9 µm, 5 µm
Biopharmaceutical separations of peptides, glycopeptides, and oligosaccharides
Biochemical analysis of sugars, nucleosides, and polar metabolites
Food and beverage testing of carbohydrates and polar compounds
Method development requiring HILIC selectivity for biomolecules
Complementary use in size-based and hydrophilic separations
| Particle Sizes |
|
|---|---|
| Pore Size | 200 Å |
| Surface Area | 200 m²/g |
| Phase/Modification | Diol |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | No |
| Carbonload | 5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 200 Diol, 1.9 µm | r219.d0. |
| ReproSil-Pur 200 Diol, 5 µm | r25.d0. |