ReproSil-Pur 300 CN is a silica-based HPLC column with cyano bonded chemistry on high-purity 300 Å silica. The wide-pore structure ensures excellent accessibility for large biomolecules such as peptides and proteins, while the cyano functionality provides moderate polarity for dipole–dipole and weak hydrophobic interactions.
This dual interaction mechanism offers complementary selectivity to traditional alkyl phases (C4, C8, C18), making ReproSil-Pur 300 CN especially useful for method development and polar biomolecular separations. The robust bonding chemistry guarantees reproducibility, sharp peaks, and long operational lifetimes.
ReproSil-Pur 300 CN combines wide-pore accessibility with polar selectivity – the dependable choice for laboratories requiring complementary separations of biomolecules in HPLC.
High-purity silica with cyano (CN) bonded chemistry
300 Å wide-pore structure for proteins, peptides, and biomolecules
Polar and dipole–dipole interactions with weak hydrophobic retention
Complementary selectivity to standard alkyl phases
Available particle size: 3 µm
Biopharmaceutical analysis of polar peptides and protein fragments
Proteomics separations requiring orthogonal selectivity
Biochemical testing of nucleosides, metabolites, and hydrophilic biomolecules
Method development as a complementary phase to C18 and C8
| Particle Size |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | CN |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | Yes |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 300 CN, 3 µm | r33.ce. |