ReproSil-Pur 300 Diol is a silica-based HPLC column with bonded diol functionality on high-purity 300 Å silica. The wide-pore design provides excellent accessibility for large biomolecules such as proteins, peptides, and oligonucleotides, while the diol chemistry offers controlled hydrophilicity for hydrophilic interaction chromatography (HILIC) and size-based separations.
Compared to bare silica, the bonded diol layer reduces strong silanol interactions, resulting in improved reproducibility, peak symmetry, and robustness. This makes ReproSil-Pur 300 Diol an excellent choice for laboratories working with polar biomolecules under both HILIC and normal-phase conditions.
ReproSil-Pur 300 Diol combines wide-pore accessibility with polar selectivity – the reliable choice for laboratories analyzing large biomolecules under HILIC or normal-phase HPLC conditions.
High-purity silica with bonded diol functionality
300 Å wide-pore structure for proteins, peptides, and large biomolecules
Suitable for HILIC and normal-phase applications
Reduced silanol activity for improved reproducibility and peak shapes
Available particle sizes: 3 µm, 5 µm
Biopharmaceutical analysis of glycoproteins, peptides, and oligonucleotides
Biochemical separations of sugars, nucleosides, and other polar biomolecules
Food and beverage testing of carbohydrates and polar compounds
Method development requiring hydrophilic interaction chromatography for large analytes
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | Diol |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | No |
| Carbonload | 3.5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 300 Diol, 3 µm | r33.d0. |
| ReproSil-Pur 300 Diol, 5 µm | r35.d0. |