ReproSil 125 SEC is a normal-phase silica column specifically optimized for size exclusion chromatography (SEC) in the molecular weight range of 5,000 – 100,000 Da. With its precisely controlled pore structure, the column enables efficient size-based separation of medium to large biomolecules while minimizing secondary interactions. Thanks to its mechanical stability and reproducible performance, ReproSil 125 SEC provides sharp peak shapes and reliable fractionation, making it an excellent choice for both analytical and preparative SEC applications in biochemistry, pharmaceutical research, and polymer analysis.
ReproSil 125 SEC combines optimized pore design with robust silica stability – the reliable choice for precise SEC separations in the 5,000 – 100,000 Da range.
Normal-phase silica optimized for size exclusion chromatography (SEC)
Molecular weight separation range: 5,000 – 100,000 Da
Accurate size-based separation with minimal secondary interactions
High mechanical stability for reproducible performance
Suitable for analytical and preparative applications
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 3 µm, 5 µm, and 10 µm particle sizes
Separation and analysis of proteins and large peptides
Characterization of biopolymers and synthetic polymers
Molecular weight determination in pharmaceutical development
Fractionation of complex biomolecular mixtures
| Particle Sizes |
|
|---|---|
| Pore Size | 125 Å |
| Phase/Modification | Diol |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil 125 SEC, 3 µm | r13.sec. |
| ReproSil 125 SEC, 5 µm | r15.sec. |
| ReproSil 125 SEC, 10 µm | r10.sec. |