ReproSil 200 SEC-2 is a silica-based HPLC column with a diol-modified surface, optimized for size exclusion chromatography (SEC) in the molecular weight range of 10,000 – 500,000 Da. The diol functionalization effectively suppresses secondary interactions, ensuring accurate size-based separations of large biomolecules and synthetic polymers. The well-defined pore structure provides high resolution for proteins, nucleic acids, and other high-molecular-weight analytes. Combined with excellent mechanical stability and reproducibility, ReproSil 200 SEC-2 is suitable for both analytical and preparative applications in biochemistry, biotechnology, and polymer research.
ReproSil 200 SEC-2 combines diol-modified silica with optimized pore design – the reliable choice for accurate and reproducible SEC separations in the 10,000–500,000 Da range.
Diol-modified silica phase for size exclusion chromatography
Molecular weight separation range: 10,000 – 500,000 Da
Suppressed secondary interactions for precise size-based separations
High mechanical stability and reproducibility
Suitable for analytical and preparative workflows
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 1.5 µm, 3 µm, 5 µm, and 10 µm particle sizes
Analysis of proteins, enzymes, and nucleic acids
Separation and characterization of biopolymers and synthetic polymers
Molecular weight distribution studies in pharmaceutical development
Fractionation of large biomolecules in biotechnology and biochemical research
| Particle Sizes |
|
|---|---|
| Pore Size | 200 Å |
| Surface Area | 200 m²/g |
| Phase/Modification | Diol |
| Particle Type | |
| Separation Mode | |
| USP Codes | |
| Endcapping | No |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil 200 SEC-2, 1.5 µm | r219.sec2. |
| ReproSil 200 SEC-2, 3 µm | r23.sec2. |
| ReproSil 200 SEC-2, 5 µm | r25.sec2. |
| ReproSil 200 SEC-2, 10 µm | r20.sec2. |