ReproSil 300 SEC

Silica-Based Column for High-Molecular-Weight Size Exclusion Chromatography

ReproSil 300 SEC is a silica-based HPLC column specifically optimized for size exclusion chromatography (SEC) in the molecular weight range of 10,000 – 1,000,000 Da. With its large pore structure, the column enables efficient fractionation and accurate size-based separation of very large biomolecules and synthetic polymers. The stationary phase provides high mechanical stability, reproducible retention, and sharp peak shapes, even for complex mixtures. This makes ReproSil 300 SEC an excellent choice for analytical and preparative SEC applications in biochemistry, biotechnology, and polymer research.

ReproSil 300 SEC combines optimized pore design with robust silica performance – the reliable choice for accurate SEC separations in the 10,000–1,000,000 Da range.

Key Features:

  • Silica-based stationary phase for size exclusion chromatography

  • Molecular weight separation range: 10,000 – 1,000,000 Da

  • Optimized pore structure for very large biomolecules and polymers

  • High reproducibility and robust performance

  • Suitable for analytical and preparative workflows

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 3 µm and 5 µm

Typical Applications:

  • Separation and analysis of proteins, enzymes, and nucleic acids

  • Characterization of biopolymers and synthetic polymers up to 1,000 kDa

  • Molecular weight distribution studies in biotechnology and pharmaceuticals

  • Fractionation of large biomolecules in biochemical and polymer research

Specifications

Request Product
Particle Sizes
  • 3 µm
  • 5 µm
Pore Size 300 Å
Phase/Modification Diol
Particle Type
Separation Mode
USP Code
Endcapping No
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil 300 SEC, 3 µm r33sec.
ReproSil 300 SEC, 5 µm r35.sec.
ReproSil 200 SEC-2 ReproSil 4000 SEC

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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